柯文哲處理吳音寧已成攻城困局 柯文哲處理吳音寧已成攻城困局

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Resultswereanalyzedstatisticallyusingafactorialarray(days,lot,monthandmedia)forthevariablegerminationandgerminationindex.Seedlings.Germinatingseeds30doldweretransferredfromthetwoculturemediausedtothesameKnudsonCvariants.,supplementedwith20g.Lˉ1ofpotatostarch.10mLof...顯示更多Resultswereanalyzedstatisticallyusingafactorialarray(days,lot,monthandmedia)forthevariablegerminationandgerminationindex.Seedlings.Germinatingseeds30doldweretransferredfromthetwoculturemediausedtothesameKnudsonCvariants.,supplementedwith20g.Lˉ1ofpotatostarch.10mLoftheculturemediawerepouredonto60mLculturevessels.DevelopmentstageswereidentifiedaccordingtoHarrisonandArditti(1978)in1)nongerminatingseeds,2)swollenseeds,3)seedsrupturingtheseedcoat,4)protocorms,5)oneleafseedling,6)twoleafplantletswithroothairs.Anaverageof25seedlingofeachstagewasevaluated.Basalbuds.Budsweredisinfectedin10%sodiumhypochloritefor10min,rinsed3-foldwithsteriledistilledwater.Bractsweretakenapartandtreatedagainwith5%and3%sodiumhypochlorite,andfinallyrinsedwithsteriledistilledwater.Afterdisinfecting,theexplant,consistingofeachdissectedbud,wasinoculatedonto25mLofKnudsonC(K-4003)culturemediumaddedwiththesupplements:BAP,NAA,potatostarch(20g.Lˉ1)andco-conutwater(10%)pouredonto125mLglassculturevessels,inordertoprovideninetreatments.CombinationsofBAP/NAAinuMineachtreatmentwereasfollow:M1(0/0.55),M2(0/1.0),M3(0.74/10.0),M4(7.4/0.056),M5(7.4/10.0),M6(7.4/5.5),M7(7.4/0),M8(7.4/10.020g.Lˉ1potatostarch)andM9(7.4/10.010%coconutwater).pHwasadjustedto5.5with1MHClandautoclavedat121°Cfor15min.Fiveexplantswereinoculatedoneachtreatment.



結果統計地被分析了使用一個階乘列陣(幾天、全部、月和媒介) 為易變的萌芽和萌芽索引。幼木。發芽種子30 d 老從二個培養基轉移了被使用到同樣Knudson C 變形, 用20 g.L..of 馬鈴薯澱粉被補充。培養基的10mL 傾吐了60 機器語言文化船。發展階段被辨認了根據哈里遜和Arditti (1978) 在1) nongerminating 的種子裡, 2) 圓鼓的種子, 3) 種子爆裂種皮, 4) protocorms, 5) 一葉子幼木, 6) 二葉子plantlets 與根毛。平均25 各個階段幼木被評估了。基礎芽。芽被消毒了在10% 鈉次氯酸鹽裡10 分鐘, 用不育的蒸餾水被漂洗3-fold 。苞分開被採取了和再被對待了與5% 和3% 鈉次氯酸鹽, 和用不育的蒸餾水最後被漂洗了。在消毒以後, explant, 包括各被解剖的芽, 被接種了25 機器語言Knudson C(K-4003)culture 媒介增加與supplements:BAP, NAA, 土豆starch(20g.L..)and 椰子water(10%) 倒125mL 玻璃文化船, 為了提供九種治療。BAP/NAA 的組合在uM 在各種治療是作為follow:M1(0/0.55), M2(0/1.0), M3(0.74/10.0), M4(7.4/0.056), M5(7.4/10.0), M6(7.4/5.5), M7(7.4/0), M8(7.4/10.0 20 g. L . . 馬鈴薯澱粉) 和M9(7.4/10.0 10% 椰子水) 。酸鹼度被調整了到5.5 以1M HCl 和被真空加熱了在121.C 15 分鐘。五explants 被接種了在各種治療。



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